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ActoFactor™ Recombinant Human Thrombopoietin

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0089
  • Description
  • Thrombopoietin (TPO) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. TPO stimulates the proliferation and maturation of megakaryocytes, and has also been shown to promote the survival, self-renewal and expansion of hematopoietic stem cells and primitive multilineage progenitors. Recombinant human TPO contains 332 amino acid residues and has a predicted molecular mass of approximately 35 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 47 kDa in SDS-PAGE.
  • Species
  • Human
  • Product Overview
  • Human THPO expressed in Sf21 insect cells
  • Sequence
  • A DNA sequence encoding the mature human TPO protein sequence (Ser 22 - Gly 353; Accession #P40225) was expressed in Sf21 insect cells.
  • Size
  • CAT# CSC-CTK0089-5 (5 μg); CAT# CSC-CTK0089-25 (25 μg)
  • Expression System
  • Sf21 insect cells
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • The biological activity of recombinant human TPO is measured in a cell proliferation assay using MO7e cells. The ED50 for this effect is typically 0.3 - 3 ng/mL.
  • Endotoxin Level
  • Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
  • Formulation
  • Recombinant human TPO is lyophilized from a 0.2 μm filtered solution in 35% acetonitrile, 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg cytokine.
  • Reconstitution
  • Reconstitute human TPO at 50 μg/mL with sterile phosphate buffered saline (PBS) containing at least 0.1% human or bovine serum albumin.
  • Storage & Stability
  • Lyophilized human TPO is stable for up to twelve months from date of receipt at -20°C to -70°C. Reconstituted human TPO can be stored under sterile conditions at 2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com