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ActoFactor™ Recombinant Human Thrombopoietin (CHO)
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Cat.No.
CSC-CTK0427
Description
Recombinant Human Thrombopoietin (rHu TPO, Mpl-ligand, MGDF) is a glycoprotein hormone produced mainly by the liver and the kidney. It stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets produced by the bone marrow in vivo. |Human recombinant Thrombopoietin produced in CHO cells consists of 332 amino acid residues with a predicted molecular mass of approximately 35 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 80±10 kDa in SDS-PAGE.
Species
Human
Product Overview
Human THPO expressed in CHO cells
Molecular Mass
80 kDa
Size
CAT# CSC-CTK0427-10 (10 μg); CAT# CSC-CTK0427-50 (50 μg)
Expression System
CHO cells
Purity
Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
rHuTPO is fully biologically active when compared to a standard. The ED50, as determined by the dose-dependant stimulation of MO7e cells, is ~1 ng/ml, corresponding to a specific activity of ~1 x 10^6 IU/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered protein solution containing 20 mM sodium citrate (pH 6.9).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu TPO should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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