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ActoFactor™ Recombinant Human Platelet derived growth factor AB

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0279
  • Description
  • Recombinant Human Platelet Derived Growth Factor-AB (rHu PDGF-AB) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Creative Bioarray's rHu PDGF-AB is a 25.5 kDa heterodimeric, non-glycosylated protein consisting of a 13.3 kDa alpha-chain and a 12.2 kDa beta-chain (234 total amino acids).
  • Species
  • Human
  • Product Overview
  • Human PDGFA expressed in E.coli
  • Molecular Mass
  • 25.5 kDa
  • Size
  • CAT# CSC-CTK0279-10 (10 μg); CAT# CSC-CTK0279-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
  • Activity
  • Creative Bioarrays human PDGF-AB is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1.0 ng/ml, corresponding to a specific activity of 1 x 10^6 IU/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • The sterile-filtered protein solution was lyophilized (from 10 mM acetic acid) with no additives.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized powder should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be further diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized PDGF-AB, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted PDGF-AB should be used immediately stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com