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ActoFactor™ Recombinant Human Placental Growth Hormone 20K
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Cat.No.
CSC-CTK0553
Description
Placental growth hormone, or growth hormone variant (GH-V), is a member of the growth hormone gene family. Its physiologic role during pregnancy remains undefined. Although extensive work has been done characterizing the signaling pathways activated by hGH, the signaling pathways which are activated by GH-V have not been characterized. Human placental GH (hPGH) replaces pituitary GH during pregnancy. hPGH is correlated to serum IGF-I in normal pregnancies and in pregnancies complicated by fetoplacental disorders. Recombinant placental human GH-20K produced in E. coli is a single, non-glycosylated polypeptide chain of 177 amino acids and a molecular mass of 20,498 Dalton (predicted pI=8.2). PGH-20K-pl is devoid of lactogenic (prolactin receptor mediated) activity characteristic to pituitary GHs.
Species
Human
Product Overview
Human GH2 expressed in E.coli
Molecular Mass
20.5 kDa
Size
CAT# CSC-CTK0553-50 (50 μg); CAT# CSC-CTK0553-250 (250 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and SEC-HPLC analysis.
Activity
N/A
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO3 previously adjusted to pH 11.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, 0.4% NaHCO3 adjusted to pH 11 to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu GH-20K-pl should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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