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ActoFactor™ Recombinant Human Placenta Growth Factor 1 (Insect)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0473
  • Description
  • Placenta growth factor (PlGF), a homodimeric glycoprotein that is homologous to vascular endothelial growth factor (VEGF), is mitogenic to endothelial cells and protects trophoblast from apoptosis. Alternative splicing of mature mRNA gives rise to three known isoforms of PlGF. PlGF is expressed by human trophoblast during normal pregnancy, however, it is not known which isoforms are produced. Recombinant human Placenta Growth Factor-1 (PIGF/PGF) produced in insect cells is a homodimeric, glycosylated polypeptide chain containing 2 x 131 amino acids and having a total molecular mass of 29.7 kDa.
  • Species
  • Human
  • Product Overview
  • Human PGF expressed in Insect cells
  • Molecular Mass
  • 29.7 kDa
  • Size
  • CAT# CSC-CTK0473-10 (10 μg); CAT# CSC-CTK0473-50 (50 μg)
  • Expression System
  • Insect cells
  • Purity
  • Greater than 90% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
  • Activity
  • Recombinant human PLGF-1 is fully biologically active when compared to a standard. The ED50 has been determined by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant human PlGF-1 can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.5 - 10 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) solution containing BSA.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, 20 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu PLGF-1 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com