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ActoFactor™ Recombinant Human Noggin (E. coli)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0548
  • Description
  • NOGGIN belongs to a group of diffusible proteins which bind to ligands of the TGF-beta family and regulate their activity by inhibiting their access to signaling receptors. It was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, NOGGIN has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of NOGGIN in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing NOGGIN in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant human NOGGIN is a 23.1 kDa non-disulfide-linked homodimer consisting of a total of 206 amino acid residues.
  • Species
  • Human
  • Product Overview
  • Human NOG expressed in E.coli
  • Molecular Mass
  • 23.1 kDa
  • Size
  • CAT# CSC-CTK0548-20 (20 μg); CAT# CSC-CTK0548-100 (100 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 95% as determined by SDS-PAGE analysis.
  • Activity
  • The ED50 was determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.05-0.08 μg/ml of NOGGIN.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized (freeze-dried), sterile powder. Lyophilized with no additives.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu NOGGIN should be used immediately or stored long- term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com