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ActoFactor™ Recombinant Human Neurotrophin 4

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0075
  • Description
  • Neurotrophin-4 (NT-4) is a member of the nerve growth factor (NGF) family of neurotrophins. It binds the TrkB receptor and acts as a survival factor for sensory neurons, although it has also been shown to sensitize cortical neurons to cell death (for a review, see 1). Together with other NGF family members NT-3 and BDNF, NT-4 has been described as a survival factor for human embryonic stem cells (hESC). Bioactive NT-3 is predicted to be a non-covalently linked homodimer. Mature recombinant NT-3 has a predicted molecular mass of approximately 13.6 kDa, with 119 amino acids following cleavage of the signal peptide and proprotein. The amino acid sequence of mature NT-3 is identical in human, mouse and rat.
  • Species
  • Human
  • Product Overview
  • Human NTF4 expressed in Sf21 insect cells
  • Sequence
  • The DNA sequence encoding the NT-3 prepropeptide3 was inserted in a baculovirus expression vector and expressed in Sf 21 insect cells.
  • Size
  • CAT# CSC-CTK0075-5 (5 μg); CAT# CSC-CTK0075-25 (25 μg)
  • Expression System
  • Sf21 insect cells
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • The ED50 of NT-4, as measured by its ability to stimulate proliferation of the TrkB-transfected cell line, BaF-TrkB-BD, is typically 5 - 15 ng/mL.
  • Endotoxin Level
  • Endotoxin level is less than 1.0 EU/μg of NT-4, as determined by the LAL method.
  • Formulation
  • Lyophilized from a sterile-filtered solution in 30% acetonitrile plus 0.1% TFA containing 50 μg of bovine serum albumin per 1 μg of NT-4.
  • Reconstitution
  • It is recommended that a stock solution at a concentration of no less than 10 μg/mL be prepared in sterile phosphate buffered saline containing at least 0.1% human or bovine serum albumin.
  • Storage & Stability
  • The lyophilized sample is stable for up to 1 year at -20°C to -70°C. Reconstituted NT-4 can be stored under sterile conditions at 2 - 8°C for 1 month or at -20°C to -70°C for 3 months in a manual defrost freezer without detectable loss of acti vity. Avoid repeated freezing and thawing.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com