- Vascular Endothelial Cell Growth Factor (VEGF) is a secreted homodimeric protein expressed by a variety of vascularized tissues. The reported activities of human VEGF include stimulation of endothelial cell growth, angiogenesis, and vascular permeability. Human VEGF is a 38.2 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains.|Human recombinant VEGF-165 produced in CHO cells is a dimeric, glycosylated polypeptide chain of 165 amino acids each and migrates as an 42 kDa in SDS-PAGE under non-reducing conditions. The VEGF has been purified by proprietary chromatographic techniques.
- Product Overview
- Human NRP1 expressed in Chinese Hamster Ovarian Cells
- CAT# CSC-CTK0403-10 (10 μg); CAT# CSC-CTK0403-50 (50 μg)
- Expression System
- Chinese Hamster Ovarian Cells
- Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.
- Determined by its ability to stimulate 3H-Thymidine incorporation in human umbilical vein endothelial cells, the ED50 for this effect was found to be 2-6 ng/ml.
- Lyophilized from a protein solution in phosphate-buffered saline (pH 7.4) with no additives.
- Please centrifuge product briefly before opening the vial. The lyophilized human VEGF should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu VEGF should be stored long-term in undiluted working aliquots at –20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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