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ActoFactor™ Recombinant Human Nerve growth factor (beta polypeptide) (CHO)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0478
  • Description
  • Recombinant Human Nerve growth factor-beta (rHu beta-NGF) is a potent neurotrophic factor, which supports the growth and survivability of nerve and/or glial cells. The active form of rHu beta-NGF is a dimer, formed by two identical subunits, which is held together by strong hydrophobic interactions. Recombinant human Nerve Growth Factor beta produced in CHO is a homodimer, glycosylated, polypeptide chain of 2 identical 119 amino acids and a molecular mass of 16,950 Dalton.
  • Species
  • Human
  • Product Overview
  • Human NGF expressed in CHO cells
  • Molecular Mass
  • 33.9 kDa
  • Size
  • CAT# CSC-CTK0478-20 (20 μg); CAT# CSC-CTK0478-100 (100 μg)
  • Expression System
  • CHO cells
  • Purity
  • Greater than 98% as determined by SDS-PAGE, FPLC and HPLC analysis.
  • Activity
  • rHu beta-NGF is fully biologically active when compared to a standard. The ED50, as calculated by its ability to stimulate chick E9 DRG neurite outgrowth, was found to be 1 x 10^6 units/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • NGF-beta was lyophilized from a 0.2 μm sterile-filter ed solution in 20 mM Tris-HCl (pH 8.0) + 0.15 M NaCl.
  • Reconstitution
  • Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into water or other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted beta-NGF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0,1% HAS or BSA). Please avoid repeated freeze / thaw cycles.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com