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ActoFactor™ Recombinant Human Nerve growth factor

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0480
  • Description
  • Pro-NGF is the pro-form of the neurotrophin nerve growth factor. Like the mature protein Pro- NGF is characterized by the cysteine knot motif consisting of three cysteine bridges. The protein predominantly exists as a non-covalently linked homodimer. Recombinant human Pro-NGF produced in E. coli is a non-glycosylated polypeptide chain of 222 amino acids and a molecular mass of 49,738 Dalton.
  • Species
  • Human
  • Product Overview
  • Human NGF expressed in E.coli
  • Molecular Mass
  • 49.7 kDa
  • Size
  • CAT# CSC-CTK0480-10 (10 μg); CAT# CSC-CTK0480-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
  • Activity
  • The activity of the protein can by measured by its stimulating effect on the proliferation of TF1 cells (Chevalier et al. 1994, Blood 83, 1479-85). EC50 = 130 ± 30 pM (TF1 cell assay).
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • The protein solution (1.2 mg/ml; 10 μg/9 μl) contai ns 50 mM sodium phosphate buffer (pH 7.2) and 150 mM sodium chloride.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. This protein solution can be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The protein solution, though stable at 15°C for up to 3 weeks, is best stored desiccated at 4°C. It should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com