- The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Human recombinant Macrophage Inducing Factor is a single, non-glycosylated, polypeptide chain containing 115 amino acids and having a molecular mass of 12.5 kDa.
- Product Overview
- Human MIER1 expressed in E.coli
- CAT# CSC-CTK0455-10 (10 μg); CAT# CSC-CTK0455-50 (50 μg)
- Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.
- Human MIF bioligical activity was determined in an agarose microduplet assay using human U937 cells. The optimal activity range found for human MIF in this assay was 0.5-1 μg/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- Sterile-filtered, lyophilized protein. MIF-Protei n was lyophilized from 10 mM sodium phosphate buffer (pH 7.5).
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu MIF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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