- Matrix metalloproteinases are a family of zinc and calcium-dependent endopeptidases that break down extracellular matrix proteins. The MMP-9 is secreted as a 92 kDa zymogen. Cleavage of ProMMP-9 results in the active enzyme, having a molecular weight of approximately 82 kDa. MMP-9 is composed of the following domains: a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP-9 is produced by the several cell types: monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells. MMP-9 is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP-9 may also play an important part in local proteolysis of the extracellular matrix and in leukocyte migration, as well as in bone osteoclastic resorption. MMP-9 cleaves type IV and type V collagens into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. MMP-9 can also degrade fibronectin but not laminin or Pz-peptide. MMP-9 defects may be a cause of susceptibility to intervertebral disc disease (IDD), also known as lumbar disk herniation (LDH). Human recombinant MMP-9 produced in E. coli is a single, non-glycosylated polypeptide chain that contains a 338 amino acids fragment (113-450; corresponding to the catalytic domain of the protein) with a total molecular mass of 42.03 kDa and that is fused with a 4.5 kDa amino- terminal hexahistidine tag.
- Product Overview
- Human MMP9 expressed in E.coli
- CAT# CSC-CTK0373-10 (10 μg); CAT# CSC-CTK0373-50 (50 μg)
- Greater than 95% as determined by SDS-PAGE analysis.
- MMP-9 protein is supplied in 20 mM Tris-HCl (pH 8.0) and 50% glycerol.
- Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. Can be used as standard in Western blots, etc.
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