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ActoFactor™ Recombinant Human Lectin, galactoside-binding, soluble, 2
- Specification
- Recommended Products
Cat.No.
CSC-CTK0580
Description
Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin-2 is an animal lectin that has been shown to interact with CD3, CD4, and CD45. It induces apoptosis of activated T-cells and T- leukemia cell lines and inhibits the protein phosphatase activity of CD45. Recombinant human Galectin-2 produced in E. coli is a single, non-glycosylated polypeptide chain of 152 amino acids (1-132 a.a.) and a molecular mass of 16.8 kDa. It is fused to a 20 amino acid His-Tag at the N-terminus and purified by proprietary chromatographic techniques.
Species
Human
Product Overview
Human LGALS2 expressed in E.coli
Molecular Mass
16.8 kDa
Size
CAT# CSC-CTK0580-25 (25 μg); CAT# CSC-CTK0580-125 (125 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
N/A
Endotoxin Level
Less than 1 EU/μg.
Formulation
0.5 mg/ml protein solution containing 20m M Tris-HCl (pH 8.0), 0.2 M NaCl, 10% glycerol and 1 mM DTT.
Reconstitution
Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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