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ActoFactor™ Recombinant Human KRIT1, ankyrin repeat containing

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0628
  • Description
  • Cerebral cavernous malformations (CCMs) are sporadically acquired or inherited vascular lesions of the central nervous system consisting of clusters of dilated thin-walled blood vessels that predispose individuals to seizures and stroke. Mutations in CCM-1, CCM-2, or CCM-3 lead to cerebral cavernous malformations, one of the most common hereditary vascular diseases of the brain. Endothelial cells within these lesions are the main disease compartments. Here, we show that adenoviral CCM-3 expression inhibits endothelial cell migration, proliferation, and tube formation while down regulation of endogenous CCM-3 results in increased formation of tube- like structures. Adenoviral CCM-3 expression does not induce apoptosis under normal endothelial cell culture conditions but protects endothelial cells from staurosporine-induced cell death. Tyrosine kinase activity profiling suggests that CCM-3 supports PDPK-1/Akt-mediated endothelial cell quiescence and survival. The CCM-1 is fused to a N-terminal His-tag (6x His). It is a 86.5 kDa protein containing 756 amino acid residues.
  • Species
  • Human
  • Product Overview
  • Human KRIT1 expressed in E.coli
  • Molecular Mass
  • 86.5 kDa
  • Size
  • CAT# CSC-CTK0628-20 (20 μg); CAT# CSC-CTK0628-100 (100 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 90% as determined by SDS-PAGE analysis.
  • Activity
  • N/A
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized from a steril-filtered protein solution in urea.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com