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ActoFactor™ Recombinant Human KIT ligand-alpha
- Specification
- Recommended Products
Cat.No.
CSC-CTK0293
Description
Stem Cell Growth Factor (SCGF) is a newly discovered hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. Human SCGF is a non-glycosylated 33.9 kDa species-specific cytokine of 305 amino acids that can support growth of primitive hematopoietic cells, and in combination of EPO or GM-CSF, it promotes proliferation of erthroid and myeloid progenitors, respectively.
Species
Human
Product Overview
Human KITLG expressed in E.coli
Molecular Mass
33.9 kDa
Size
CAT# CSC-CTK0293-10 (10 μg); CAT# CSC-CTK0293-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analyses. Endotoxin level is less than
Activity
Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect is 2.5 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered protein solution with no additives.
Reconstitution
Please centrifuge product briefly before opening the vial. The lyophilized SCGF is soluble in water and most aqueous buffers. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1 mg/ml. It is recommended that further dilutions be made into buffer containing carrier protein (HSA or BSA) or medium containing serum.
Storage & Stability
The lyophilized SCGF, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted SCGF should be used immediately or stored long-term in undiluted working aliquots at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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