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ActoFactor™ Recombinant Human Interleukin 4 (CHO)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0226
  • Description
  • Human Interleukin-4 (rHu IL-4) is a 15 kDa protein containing 130 amino acid residues. rHu IL-4 is a potent lymphoid cell growth factor, which stimulates the growth and survivability of certain B-cells and T-Cells.|Human recombinant Interleukin-4 produced in CHO cells is a single, non- glycosylated polypeptide chain of 129 amino acids having a molecular mass of 14 kDa. The glycosylated IL-4 migrates as a 18 kDa band in SDS-PAGE analysis. The protein has been purified by using proprietary chromatographic techniques.
  • Species
  • Human
  • Product Overview
  • Human IL4 expressed in CHO cells
  • Molecular Mass
  • 14 kDa
  • Size
  • CAT# CSC-CTK0226-10 (10 μg); CAT# CSC-CTK0226-50 (50 μg)
  • Expression System
  • CHO cells
  • Purity
  • Greater than 97% as determined by SDS-PAGE and HPLC analysis.
  • Activity
  • Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 0.05-0.2 ng/ml.
  • Endotoxin Level
  • N/A
  • Formulation
  • Lyophilized from sterile-filtered protein solution in phosphate-buffered saline (pH 7.4).
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
  • Storage & Stability
  • The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze/thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com