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ActoFactor™ Recombinant Human Interleukin 3 (colony-stimulating factor, multiple) (E. coli)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0219
  • Description
  • Recombinant Human Interleukin-3 (rhu IL-3) is a species specific colony stimulating factor, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. Human IL-3 is a 15.0 kDa single, non-glycosylated polypeptide chain containing 133 amino acid residues.
  • Species
  • Human
  • Product Overview
  • Human IL3 expressed in E.coli
  • Molecular Mass
  • 15.0 kDa
  • Size
  • CAT# CSC-CTK0219-10 (10 μg); CAT# CSC-CTK0219-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by SDS-PAGE, RP-HPLC and AEX-FLPC analysis.
  • Activity
  • The ED50 as determined by the dose-dependent stimulation of TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of 1 x 10^7 units/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized from a sterile-filtered, concentrated (1 mg/ml) protein solution in water containing 0.3 mg/ml NaHCO .
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized IL-3, though stable at room temperature for up to 3 weeks, is best stored at - 20°C. Reconstituted IL-3 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0,1% HSA or BSA). Please avoid repeated freeze / thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com