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ActoFactor™ Recombinant Human Interleukin 3 (colony-stimulating factor, multiple)
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Cat.No.
CSC-CTK0221
Description
IL-3 is a potent growth factor involved in a variety of cell activities such as cell growth, differentiation and apoptosis. It is involved in several biological responses such as proliferation and differentiation of a broad range of hematopoietic progenitor cells into erythrocytes, granulocytes, monocytes, megakaryocytic and mast cells. IL-3 also induces the production of several enzymes involved in cellular metabolism, differentiation, and DNA/RNA metabolism. IL-3 is produced by activated T-lymphocytes, keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. The biological activity of IL-3 is mediated through a specific cell surface receptor that is composed of alpha and beta subunits. Alpha subunit is responsible for the binding and beta subunit transmits signals across the plasma membrane. IL-3 is known to activate three signalling pathways: JAK/STAT, RAS/RAF/MAP kinase, and the PI-3 kinase/PKB pathways. IL-3 is also implicated in the pathogenesis of several diseases such as asthma, atherosclerosis and multiple sclerosis. The recombinant protein has been widely used in clinical practice, in the treatment of leukemia and for therapy of patients with bone marrow deficiency function. Recombinant human IL-3 is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture. Recombinant human IL-3 contains 133 amino acids and a 16 a.a. histidine-based tag for a total length of 149 a.a. and has a predicted molecular mass of 17.2 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 20-23 kDa in SDS-PAGE.
Species
Human
Product Overview
Human IL3 expressed in Endosperm tissue of barley grain (Hordeum vulgare)
Molecular Mass
17.2 kDa
Size
CAT# CSC-CTK0221-10 (10 μg); CAT# CSC-CTK0221-50 (50 μg)
Expression System
Endosperm tissue of barley grain (Hordeum vulgare)
Purity
Greater than 95% as determined by SDS-PAGE analysis. Preparation carries no pyrogenic or pro-
Activity
Each batch is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of recombinant human IL-3 was determined by its dose-dependent effects on the proliferation of TF-1 cells. The ED50 value for this effect is typically 5 x 10^6 units/mg. Optimal concentration should be determined for specific applications and cell lines.
Endotoxin Level
Less than 0.05 EU/μg.
Formulation
Lyophilized from a concentrated, sterile protein solution in PBS (pH 7.2).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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