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ActoFactor™ Recombinant Human Interleukin 17F (HEK)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0263
  • Description
  • IL-17F, a member of the IL-17 family of structurally related cytokines, has been shown to stimulate proliferation and activation of T-cells and PBMCs. IL-17F shares sequence similarity with IL-17. IL-17F is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL-6, IL-8, and CSF2/GM-CSF. IL-17F inhibits the angiogenesis of endothelial cells and induce endothelial cells to produce IL-2, TGFB1/TGFB, and monocyte chemoattractant protein-1. IL-17F induces stromal cells to produce proinflammatory and hematopoietic cytokines. Intestinal IL-17F gene expression is increased in active CD. IL-17A and IL-17F alleles influence the susceptibility to and pathophysiological features of ulcerative colitis independently. IL-17F and MIF gene polymorphisms are significantly associated with the development of functional dyspepsia. The initiation of IL-17F/IL-17R signaling pathway requires the receptor ubiquitination by TRAF-6. IL-17F induces expression of IFN-gamma-inducible protein 10 (IP-10) by activating Raf1- mitogen-activated protein kinase 1/2-extracellular-regulated kinase 1/2-p90 ribosomal S6 kinase-cyclic AMP response element-binding protein signaling pathway.IL-17F also regulates cartilage matrix turnover and inhibits angiogenesis. Recombinant human IL-17F produced in HEK cells is a glycosylated homodimer, having a total molecular weight of 38 kDa. It has been purified by proprietary chromatographic techniques.
  • Species
  • Human
  • Product Overview
  • Human IL17F expressed in HEK cells
  • Molecular Mass
  • 38 kDa
  • Size
  • CAT# CSC-CTK0263-10 (10 μg); CAT# CSC-CTK0263-50 (50 μg)
  • Expression System
  • HEK cells
  • Purity
  • Greater than 95% as determined by SDS-PAGE analysis.
  • Activity
  • rHu IL-17F is fully biologically active when compared to standards. The specific activity was determined by the dose-dependent induction of IL-6 secretion from NHDF adult fibroblasts, and is typically 100-500 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered, white, lyophilized (freeze-dried)powder. Lyophilized from a concentrated protein solution (1 mg/ml) in 1xPBS.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-17F should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com