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ActoFactor™ Recombinant Human Interleukin 17A (E. coli)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0258
  • Description
  • IL-17 is a potent proinflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. As interleukin-17 shares properties with IL-1 and TNF-alpha, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. Interleukin-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation. Recombinant human IL-17 produced in E. coli is a homodimeric, non-glycosylated polypeptide chain containing of 264 amino acids and a molecular mass of 30,250 Dalton.
  • Species
  • Human
  • Product Overview
  • Human IL17A expressed in E.coli
  • Molecular Mass
  • 30.25 kDa
  • Size
  • CAT# CSC-CTK0258-25 (25 μg); CAT# CSC-CTK0258-125 (125 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
  • Activity
  • The ED50 as determined by the dose-dependent induction of IL-6 in Hs68 cell line and was found to be ~2 ng/ml, corresponding to a specific activity of 5 x 10^5 Units/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) protein solution in 20 mM citrate, 0.1 M NaCl(pH 4.5).
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-17 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com