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ActoFactor™ Recombinant Human Interleukin-13

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0050
  • Description
  • Human Interleukin 13 (IL-13) is produced by activated Th0, Th1, Th2 and CD8 T cells. IL-13 is a pleiotropic immune regulatory cytokine that promotes growth of human B cells; induces expression of germ line Ce transcripts, directs na?ve B cells to switch to the synthesis of IgE and IgG4, and induces expression of CD23 and ClassII-MHC. In monocytes, IL-13 down-regulates FcgR expression; inhibits synthesis of proinflammatory cytokines, including TNF-a, IL-1b, IL-6 and IL-8; induces monocyte/macrophage fusion and mannose receptor expression. IL-13 inhibits the synthesis of IL-12, nitric oxide synthase and cyclooxigenase-2. The human IL-13 cDNA encodes a 132 amino acid protein containing a 20 amino acid signal peptide. IL-13 binds to a heterodimeric receptor formed by the IL-13Ra and the IL-4Rb. Several high affinity IL-13 binding proteins have been reported.
  • Species
  • Human
  • Product Overview
  • Human IL13 expressed in E.coli
  • Sequence
  • A DNA sequence corresponding to amino acid residues Gly21 to Asn132 of the human IL-13 cDNA, containing Gly at position 61 and a Gln insertion at position 98, was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0050-5 (5 μg); CAT# CSC-CTK0050-25 (25 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • Activity was determined in a cell proliferation assay using a human factor-dependent cell line, TF-1 and the ED50 for this effect was typically 3 - 6 ng/mL.
  • Endotoxin Level
  • Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
  • Formulation
  • Lyophilized from a 0.2 μm filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
  • Reconstitution
  • It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 5 mg /mL of the cytokine.
  • Storage & Stability
  • Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted hIL-13 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com