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ActoFactor™ Recombinant Human Interleukin 12 (HEK)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0247
  • Description
  • Recombinant Human Interleukin-12 (rHu IL-12) is a regulatory protein produced by activated B- lymphocytes and macrophages. Human IL-12 produced in HEK cells is a heterodimeric, glycosylated protein with a molecular weight of 57 kDa.
  • Species
  • Human
  • Product Overview
  • Human IL12B expressed in HEK cells
  • Molecular Mass
  • 57 kDa
  • Size
  • CAT# CSC-CTK0247-10 (10 μg); CAT# CSC-CTK0247-50 (50 μg)
  • Expression System
  • HEK cells
  • Purity
  • Greater than 95% as determined by SDS-PAGE analysis.
  • Activity
  • Specific activity was determined by the dose-dependent release of IFN-gamma from the human NK92 cell line in presence of 20 ng/ml rat IL-2 and is typically 0.1 - 0.5 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized from a sterile-filtered protein solution (1 mg/ml) in 1x PBS buffer.
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. The lyophilized IL-12 should be reconstituted in sterile, ultra-pure water to a concentration of 0.2 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-12 should be stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com