- Recombinant Human Interleukin-12 (rHu IL-12) is produced by activated B-lymphocytes, macrophages and connective tissue type mast cells. It is a potent regulator of cell mediated immune responses and induces IFN-γ production by NK and T cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-γ producing Th1 cells. Recombinant human IL-12 produced in CHO cells is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).
- Product Overview
- Human IL12B expressed in CHO cells
- CAT# CSC-CTK0248-10 (10 μg); CAT# CSC-CTK0248-50 (50 μg)
- Expression System
- CHO cells
- Greater than 98% as determined by SDS-PAGE analysis.
- Assay #1: Determined by its ability to induce IFN-γ production from NK cells co-stimulated with IL-18. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of Greater than 1×10^6 IU/mg.
Assay #2: Determined by its ability to increase IFN-γ production by anti-TCR mAb-stimulated PBMCs. The expected ED50 for this effect is 4.0-8.0 ng/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- Sterile filtered through a 0.2 micron filter and lyophilized from 0.5x PBS (pH 7.2).
- Please centrifuge product briefly before opening the vial. The lyophilized IL-12 should be reconstituted in sterile 1x PBS (pH 7.2-7.4) to a concentration of 0.2 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-12 should be stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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