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ActoFactor™ Recombinant Human Interleukin 12 (CHO)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0248
  • Description
  • Recombinant Human Interleukin-12 (rHu IL-12) is produced by activated B-lymphocytes, macrophages and connective tissue type mast cells. It is a potent regulator of cell mediated immune responses and induces IFN-γ production by NK and T cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-γ producing Th1 cells. Recombinant human IL-12 produced in CHO cells is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).
  • Species
  • Human
  • Product Overview
  • Human IL12B expressed in CHO cells
  • Molecular Mass
  • 75 kDa
  • Size
  • CAT# CSC-CTK0248-10 (10 μg); CAT# CSC-CTK0248-50 (50 μg)
  • Expression System
  • CHO cells
  • Purity
  • Greater than 98% as determined by SDS-PAGE analysis.
  • Activity
  • Assay #1: Determined by its ability to induce IFN-γ production from NK cells co-stimulated with IL-18. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of Greater than 1×10^6 IU/mg. Assay #2: Determined by its ability to increase IFN-γ production by anti-TCR mAb-stimulated PBMCs. The expected ED50 for this effect is 4.0-8.0 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile filtered through a 0.2 micron filter and lyophilized from 0.5x PBS (pH 7.2).
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. The lyophilized IL-12 should be reconstituted in sterile 1x PBS (pH 7.2-7.4) to a concentration of 0.2 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-12 should be stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com