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ActoFactor™ Recombinant Human Interleukin 11 (Pichia)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0246
  • Description
  • IL-11 is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL-6ST (gp130). IL-11 is shown to stimulate the T-cell- dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Recombinant human IL-11 produced in Pichia pastoris is a single, non-glycosylated, Polypeptide chain of 177 amino acids (it differs from the 178 amino acid length of the native IL-11 only in lack of the N-terminal praline residue) and a molecular mass of 19 kDa. It has been purified by proprietary chromatographic techniques.
  • Species
  • Human
  • Product Overview
  • Human IL11 expressed in Pichia pastoris
  • Molecular Mass
  • 19 kDa
  • Size
  • CAT# CSC-CTK0246-10 (10 μg); CAT# CSC-CTK0246-50 (50 μg)
  • Expression System
  • Pichia pastoris
  • Purity
  • Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
  • Activity
  • rHu IL-11 is fully biologically active when compared to a standard. The ED50, as determined by the dose-dependent stimulation of the proliferation of murine 7TD1, was found to be less then 0.2 ng/ml, corresponding to a Specific Activity of 8,000,000 units/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered protein solution was lyophilized after extensive dialysis against 20 mM PBS (pH 7.0) and 2% glycine buffer.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-11 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com