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ActoFactor™ Recombinant Human Interleukin 10 (E. coli)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0243
  • Description
  • Recombinant Human Interleukin-10 (rHu IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.6 kDa protein of 161 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of rHu IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production.|Human recombinant IL-10 produced in E. coli is a non-covalent dimer of 2 polypeptide chains containing 161 amino acids each and having a molecular mass of 18606.29 Dalton. It has been purified by using proprietary chromatographic techniques.
  • Species
  • Human
  • Product Overview
  • Human IL10 expressed in E.coli
  • Molecular Mass
  • 18.6 kDa
  • Size
  • CAT# CSC-CTK0243-10 (10 μg); CAT# CSC-CTK0243-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and HPLC analysis.
  • Activity
  • Creative Bioarray's rHu IL-10 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependant stimulation of mouse MC-9 cells was found to be <2 ng/ml, corresponding to a Specific Activity of 5 x 10^5 IU/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized from a concentrated (1 mg/ml), sterile-filtered protein solution in PBS (pH 7.4) with no additives.
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at –20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted rHu IL-10 should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com