Online Inquiry & Support

USA: 1-631-626-9181   Fax: 1-631-614-7828
Europe: 44-207-097-1828
Email: info@creative-bioarray.com

ActoFactor™ Recombinant Human Interleukin 10

Bookmark and Share
  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0082
  • Description
  • Human Interleukin 10 (IL-10) is a species cross-reactive cytokine produced by CD4+ as well as by some CD8+ T cells. In addition, human B cells, EBV-transformed lymphoblastoid cell lines and monocytes can produce IL-10 upon activation. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation , Ig secretion and class II MHC expression. IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development. As an immunosuppressant, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by downregulating class II MHC expression. It inhibits IFN-g synthesis and Th1 differentiation in the mouse system. It can suppress the production of numerous cytokines including TNF-a, IL-1 and IL-6, as well as the synthesis of superoxide anion, reactive oxygen intermediates (ROI), and reactive nitrogen intermediates (RNI) by activated monocytes/macrophages. Recombinant human IL-10 containing 160 amino acid residues has a predicted molecular mass of approximately 18.6 kDa.
  • Species
  • Human
  • Product Overview
  • Human IL10 expressed in Sf21
  • Sequence
  • A DNA sequence encoding the mature human IL-10 protein was expressed in Sf21 insect cells.
  • Size
  • CAT# CSC-CTK0082-5 (5 μg); CAT# CSC-CTK0082-25 (25 μg)
  • Expression System
  • Sf21
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • Activity was determined in a cell proliferation assay using the murine mast cell line, MC/9 and the ED50 using this assay was typically 0.5 – 1.0 ng/ml.
  • Endotoxin Level
  • Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
  • Formulation
  • Lyophilized from a sterile-filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
  • Reconstitution
  • It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/ml of the cytokine.
  • Storage & Stability
  • The lyophilized sample is stable for greater than six months at -20°C to -70°C. Reconstituted IL-10 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Price Inquiry

Please input "bioarray" as verification code.

SEARCH

tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

  

SEARCH

Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
Ordering Information
    • Payment methods we support:
      ✭ Invoice / Purchase Order
      ✭ Credit card
Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com