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ActoFactor™ Recombinant Human Interferon, gamma
- Specification
- Recommended Products
Cat.No.
CSC-CTK0191
Description
IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN- gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN- gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells. Recombinant human IFN-gamma produced in plant is a 18.8 kDa protein containing 143 amino acid residues and contains a 16 a.a. histidine-based tag for a total length of 159 a.a. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 25 and 27 kDa in SDS-PAGE.
Species
Human
Product Overview
Human IFNG expressed in Endosperm tissue of barley grain (Hordeum vulgare)
Molecular Mass
18.8 kDa
Size
CAT# CSC-CTK0191-100 (100 μg); CAT# CSC-CTK0191-500 (500 μg)
Expression System
Endosperm tissue of barley grain (Hordeum vulgare)
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
The ED50 as determined by the dose-dependent cytostatic effects of serial dilutions of IFN- gamma in the presence of TNF-alpha on HT-29 (human carcinoma) cells is 0.12 ng/ml corresponding to a specific activity of 8.5 x 10^6 units/mg. The optimal concentration should be determined for specific applications and cell lines.
Endotoxin Level
Less than 0.05 EU/μg.
Formulation
Sterile filtered through a 0.2 μm filter. Lyophilized from PBS (pH 7.4).
Reconstitution
Please centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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