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ActoFactor™ Recombinant Human Interferon, gamma

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0047
  • Description
  • Interferon-g (IFN-g), also known as Type II interferon or immune interferon, is a highly species-specific pleiotropic cytokine produced primarily by T and NK cells. Natural mature IFN-g exists as non-covalently-linked homodimers with different levels of glycosylation. IFN-g exerts antiviral, antiproliferative, immunoregulatory and proinflammatory activities. It induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. IFN-g also augments Th1 cell expansion and may be required for Th1 cell differentiation. IFN-g binds to a heterodimer cell surface receptor that when stimulated activates the JAK-STAT pathway. Recombinant human IFN-g is a 17 kDa protein containing 144 amino acid residues.
  • Species
  • Human
  • Product Overview
  • Human IFNG expressed in E.coli
  • Sequence
  • A DNA sequence encoding the mature human IFN-g protein was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0047-100 (100 μg); CAT# CSC-CTK0047-500 (500 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • Activity was determined by measuring the antiviral effect using HeLa cells infected with EMC virus and the ED50 using this assay was typically 8-15 units/mL or 0.8-1.5 ng/mL, giving a specific activity of approximately 1 x 10^7 units/mg protein.
  • Endotoxin Level
  • Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
  • Formulation
  • Lyophilized from a 0.2 μm filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
  • Reconstitution
  • It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/ml of the cytokine.
  • Storage & Stability
  • The lyophilized sample is stable for greater than six months at -20°C to -70°C. Reconstituted IFN-gcan be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com