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ActoFactor™ Recombinant Human Interferon, gamma
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Cat.No.
CSC-CTK0047
Description
Interferon-g (IFN-g), also known as Type II interferon or immune interferon, is a highly species-specific pleiotropic cytokine produced primarily by T and NK cells. Natural mature IFN-g exists as non-covalently-linked homodimers with different levels of glycosylation. IFN-g exerts antiviral, antiproliferative, immunoregulatory and proinflammatory activities. It induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. IFN-g also augments Th1 cell expansion and may be required for Th1 cell differentiation. IFN-g binds to a heterodimer cell surface receptor that when stimulated activates the JAK-STAT pathway. Recombinant human IFN-g is a 17 kDa protein containing 144 amino acid residues.
Species
Human
Product Overview
Human IFNG expressed in E.coli
Sequence
A DNA sequence encoding the mature human IFN-g protein was expressed in E. coli.
Size
CAT# CSC-CTK0047-100 (100 μg); CAT# CSC-CTK0047-500 (500 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
Activity was determined by measuring the antiviral effect using HeLa cells infected with EMC virus and the ED50 using this assay was typically 8-15 units/mL or 0.8-1.5 ng/mL, giving a specific activity of approximately 1 x 10^7 units/mg protein.
Endotoxin Level
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/ml of the cytokine.
Storage & Stability
The lyophilized sample is stable for greater than six months at -20°C to -70°C. Reconstituted IFN-gcan be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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