- Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFN-alpha is an acid stable molecule that signals through IFN-alpha/betaR, which is also used by IFN-beta. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. Recombinant human IFN-beta 1b produced in E. coli is a non-glycosylated 18.5 kDa protein containing 165 amino acid residues.
- Product Overview
- Human IFNA1 expressed in E.coli
- CAT# CSC-CTK0184-10 (10 μg); CAT# CSC-CTK0184-50 (50 μg)
- Greater than 98% as determined by SDS-PAGE, FPLC and HPLC analysis.
- Interferon beta-1b is fully biologically active when compared to a standard. The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 3.2 x 10^7 IU/mg.
- Endotoxin Level
- Less than 1 EU/μg.
- Lyophilized from a sterile-filtered protein solution containing HSA and dextrose.
- Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. Further dilutions should be made into buffer containing carrier protein or medium containing serum.
- Storage & Stability
- The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted IFN-beta should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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