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ActoFactor™ Recombinant Human Interferon alpha-2a

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0179
  • Description
  • Human Interferon-alpha 2a is an interferon class I produced by the cells of the innate immune system as a part of the defense response to foreign agents such as viruses, parasites and tumor cells. The human alpha-interferon family displays broad spectrum antiviral, antiproliferative and immunomodulatory activities on a variety of cell types. Interferon-alpha 2a (IFNa-2a), one of the subtypes, is a protein consisting of 165 amino acids. Its three-dimensional loop structure is maintained by two disulfide bridges. It has an approximate molecular weight of 20 kDa. Recombinant human IFN-alpha 2a is a polypeptide single chain containing 171 amino acids with a predicted molecular mass of 20.1 kDa. It contains a His-tag at the C-terminal end. Human recombinant IFN-alpha 2a expressed in Nicotiana benthamiana is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product does not contain any animal–derived components or impurities.
  • Species
  • Human
  • Product Overview
  • Human IFNA2 expressed in Nicotiana benthamiana
  • Molecular Mass
  • 20.1 kDa
  • Size
  • CAT# CSC-CTK0179-10 (10 μg); CAT# CSC-CTK0179-50 (50 μg)
  • Expression System
  • Nicotiana benthamiana
  • Purity
  • Greater than 97% as determined by SDS-PAGE analysis.
  • Activity
  • The specific activity was determined by a quantitative gene reported bioassay using human Type I interferon-sensitive cells. The activity is <8 x 10^8 units/mg in reference to a viral resistance assay using bovine kidney MDBK cells.
  • Endotoxin Level
  • Less than 0.04 EU/μg.
  • Formulation
  • Lyophilized from a concentrated, sterile protein solution in 0.05 M Tris-HCl buffer (pH 7.4) with 0.01% SDS.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com