- GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. Human Recombinant Growth Hormone produced in HEK cells is a non-glycosylated monomer, having a total molecular weight of 22 kDa. It has been purified using proprietary chromatographic techniques.
- Product Overview
- Human GH1 expressed in HEK cells
- CAT# CSC-CTK0543-10 (10 μg); CAT# CSC-CTK0543-50 (50 μg)
- Expression System
- HEK cells
- Greater than 95% as determined by SDS-PAGE analysis.
- The activity was determined by the dose dependent stimulation of the proliferation of rat lymphoma line Nb2-11 cells (prolactin indicator cell line) and is typically 0.4-2 ng/ml.
- White (freeze-dried) powder. Lyophilized from a sterile-filtered, concentrated protein solution (1 mg/ml) in 1xPBS.
- Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted GH should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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