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ActoFactor™ Recombinant Human Fms-related tyrosine kinase 3 (Insect)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0519
  • Description
  • Recombinant Human flt3-Ligand (rHu flt3-Ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells. rhu flt3-Ligand is a soluble 17.6 kDa polypeptide containing 155 amino acid residues, which comprises the extracellular domain of the transmembrane flt3-Ligand protein.
  • Species
  • Human
  • Product Overview
  • Human FLT3 expressed in Sf9-insect cells
  • Molecular Mass
  • 17.6 kDa
  • Size
  • CAT# CSC-CTK0519-10 (10 μg); CAT# CSC-CTK0519-50 (50 μg)
  • Expression System
  • Sf9-insect cells
  • Purity
  • Greater than 95% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
  • Activity
  • Recombinant Flt3-Ligand is fully biologically active when compared to a standard. The ED50, calculated by the dose-dependant stimulation of the proliferation of human MUTZ-2cells, is < 3.0 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • The sterile filtered solution was lyophilized with no additives.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20 C° for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu flt3-ligand-Sf9 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com