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ActoFactor™ Recombinant Human Fms-related tyrosine kinase 3 (HEK)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0520
  • Description
  • Recombinant human flt-3 Ligand stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells. Flt-3 ligand also has a tyrosine-protein kinase activity and synergizes with other CSFs and interleukins to induce growth and differentiation. Recombinant human flt-3 Ligand produced in HEK293 cells is a glycosylated polypeptide chain and migrates as a diffuse band on SDS-PAGE due to heterogeneous glycosylation at 30 kDa.
  • Species
  • Human
  • Product Overview
  • Human FLT3 expressed in HEK293 cells
  • Molecular Mass
  • 17.6 kDa
  • Size
  • CAT# CSC-CTK0520-10 (10 μg); CAT# CSC-CTK0520-50 (50 μg)
  • Expression System
  • HEK293 cells
  • Purity
  • Greater than 95% as determined by SDS-PAGE and HPLC analysis.
  • Activity
  • The ED50 as determined by the dose dependent proliferation of human OCI-AML5 cells is 0.5 to 1.0 ng/ml, corresponding to a specific activity of Greater than 1 x 10^6 units/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a protein solution with no additives.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20 C° for future use.
  • Storage & Stability
  • The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com