Featured Products
- HCC-78
- HDLM-2
- DOHH-2
- L-540
- MX-1
- NALM-6
- NB-4
- CAL-51
- SNB-19
- KYSE-520
- MKN-45
- BA/F3
- MS-5
- HCEC-B4G12
- NK-92
- PA-TU-8988S
- MONO-MAC-1
- PA-TU-8902
- Human Microglia
- Human Hepatic Stellate Cells
- Human Skeletal Muscle Cells (DMD)
- Human Schwann Cells
- Human Oral Keratinocytes (HOK)
- Human Cardiomyocytes
- Human Small Intestinal Epithelial Cells
- Human Colonic Epithelial Cells
- Human Intestinal Fibroblasts
- Primary Human Large Intestine Microvascular Endothelial Cells
- Human Small Intestinal Microvascular Endothelial Cells
- Human Retinal Pigment Epithelial Cells
- Human Hepatocytes
- Cynomolgus Monkey Lung Microvascular Endothelial Cells
- Cynomolgus Monkey Vein Endothelial Cells
- C57BL/6 Mouse Primary Mammary Epithelial Cells
- C57BL/6 Mouse Vein Endothelial Cells
- Rat Primary Kidney Epithelial Cells
- Rat Gingival Epithelial Cells
- Rabbit Lung Endothelial Cells
Our Promise to You
Guaranteed product quality, expert customer support
ActoFactor™ Recombinant Human Fms-related tyrosine kinase 3 (HEK)
- Specification
- Recommended Products
Cat.No.
CSC-CTK0520
Description
Recombinant human flt-3 Ligand stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells. Flt-3 ligand also has a tyrosine-protein kinase activity and synergizes with other CSFs and interleukins to induce growth and differentiation. Recombinant human flt-3 Ligand produced in HEK293 cells is a glycosylated polypeptide chain and migrates as a diffuse band on SDS-PAGE due to heterogeneous glycosylation at 30 kDa.
Species
Human
Product Overview
Human FLT3 expressed in HEK293 cells
Molecular Mass
17.6 kDa
Size
CAT# CSC-CTK0520-10 (10 μg); CAT# CSC-CTK0520-50 (50 μg)
Expression System
HEK293 cells
Purity
Greater than 95% as determined by SDS-PAGE and HPLC analysis.
Activity
The ED50 as determined by the dose dependent proliferation of human OCI-AML5 cells is 0.5 to 1.0 ng/ml, corresponding to a specific activity of Greater than 1 x 10^6 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a protein solution with no additives.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20 C° for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Customer Support & Price Inquiry
