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ActoFactor™ Recombinant Human Fibroblast growth factor (acidic) intracellular binding protein (Insect)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0140
  • Description
  • aFGF is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and it is a potent inducer of angiogenesis. It binds to heparin, which potentiates its biological activity and protects it from proteolysis. aFGF is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages, and has also chemotactic and mitogenic activities. It was originally named acidic fibroblast growth factor based upon its chemical properties and to distinguish it from basic fibroblast growth factor. Recombinant Human aFGF (FGF-1) produced in Sf9 insect cells is a single, glycosylated polypeptide chain of 140 amino acids and a molecular mass of 15,803 Dalton.
  • Species
  • Human
  • Product Overview
  • Human FIBP expressed in Sf9-insect cells
  • Molecular Mass
  • 15.8 kDa
  • Size
  • CAT# CSC-CTK0140-10 (10 μg); CAT# CSC-CTK0140-50 (50 μg)
  • Expression System
  • Sf9-insect cells
  • Purity
  • Greater than 95% as determined by SDS-PAGE, FPLC and HPLC analysis.
  • Activity
  • Recombinant Human aFGF is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <10 ng/ml, corresponding to a specific activity of 10^5 Units/mg.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered liquid formulation. The sterile protein solution (1.8 mg/ml) contains 20 mM TrisHCl (pH 7.9), 100 mM KCl, 0.2 mM DTT and 20% glycerol.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The recombinant aFGF-Sf9, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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    • USA
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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com