- KGF is a potent specific mitogen for many epithelial cells but not for fibroblasts and endothelial cells. KGF is thought to play an important role in the paracrine growth control of normal epithelial cells. KGF stimulates the proliferation of primary and secondary human keratinocytes to the same extent as EGF. Recombinant human KGF-1 (FGF-7) produced in E. coli is a single, non- glycosylated polypeptide chain of 164 amino acids and a molecular mass of 18,995 Dalton.
- Product Overview
- Human FGF7 expressed in E.coli
- CAT# CSC-CTK0363-10 (10 μg); CAT# CSC-CTK0363-50 (50 μg)
- Greater than 96% as determined by SDS-PAGE and RP-HPLC analysis.
- Recombinant human Keratinocye Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake), is 1 x 10^5 units/mg.
- Endotoxin Level
- Less than 1 EU/μg.
- White (freeze-dried) powder. Lyophilized from a sterile-filtered protein solution in 20 mM phosphate buffer (pH 8.0), 1 M NaCl.
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu KGF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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