- FGF-23 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-23 inhibits renal tubular phosphate transport. This gene was identified by its mutations associated with autosomal dominant hypophosphatemic rickets (ADHR), an inherited phosphate wasting disorder. Abnormally high level expression of FGF-23 was found in oncogenic hypophosphatemic osteomalacia (OHO), a phenotypically similar disease caused by abnormal phosphate metabolism. Mutations in FGF-23 have also been shown to cause familial tumoral calcinosis with hyperphosphatemia. Human recombinant FGF-23 produced in E. coli is a 25.5 kDa globular protein containing 228 amino acid residues.
- Product Overview
- Human FGF23 expressed in E.coli
- CAT# CSC-CTK0174-20 (20 μg); CAT# CSC-CTK0174-100 (100 μg)
- Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
- Endotoxin Level
- Less than 1 EU/μg.
- Sterile-filtered through a 0.2 micron filter and lyophilized from a protein solution in 10 mM sodium acetate (pH 6.0) and 0.1 M arginine.
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultrapure water to a concentration of 0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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