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ActoFactor™ Recombinant Human Fibroblast growth factor 2 (basic) (147 aa)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0137
  • Description
  • Recombinant Human basic Fibroblast Growth Factor (rHu bFGF) is a heparin binding growth factor, which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. rHu bFGF also exerts a potent angiogenic activity in vivo. Recombinant human FGF-b (FGF-2) produced in E. coli is a single, non-glycosylated polypeptide chain of 147 amino acids having a molecular mass of 16,539 Dalton.
  • Species
  • Human
  • Product Overview
  • Human FGF2 expressed in E.coli
  • Molecular Mass
  • 16.54 kDa
  • Size
  • CAT# CSC-CTK0137-10 (10 μg); CAT# CSC-CTK0137-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and HPLC analysis.
  • Activity
  • The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells, was found to be 0.01-0.17 ng/ml, corresponding to a specific activity of between 5.8 x 10^6 and 1 x 10^7 units/mg.
  • Endotoxin Level
  • N/A
  • Formulation
  • FGF-2 was lyophilized from a concentrated (1 mg/ml) sterile protein solution containing 10 mM sodium phosphate buffer (pH 8).
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com