- CTLA-4 (CD152) is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have also been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds the CD28 ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind the CD28 ligand with high affinity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization. |Human recombinant CTLA-4 produced in insect cells is a homodimeric, glycosylated polypeptide chain( having a total molecular mass of 80 kDa. Each subunit (40 kDa) is fused via a polypeptide linker to the Fc portion of human IgG1. It has been purified by using proprietary chromatographic techniques.
- Product Overview
- Human CTLA4 expressed in Sf9 insect cells
- Molecular Mass
- 2 x 40 kDa
- CAT# CSC-CTK0315-25 (25 μg); CAT# CSC-CTK0315-125 (125 μg)
- Expression System
- Sf9 insect cells
- Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
- Lyophilized from a sterile-filtered protein solution with no additives.
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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