- CTGF is a member of the CCN family of secreted cysteine rich regulatory proteins and is the major mitogenic and chemoattractant protein produced by umbilical vein and vascular endothelial cells. CTGF stimulates the proliferation and differentiation of chondrocytes, induces angiogenesis, promotes cell adhesion of fibroblasts, endothelial, and epithelial cells, and binds to IGF, TGF beta1, and BMP-4. Cell migration and adhesion are signaled through binding to specific cell surface integrins and to heparin sulfate proteoglycans CTGF (98 a.a.), a lower molecular weight isoform containing the C-terminal portion of the full length CTGF protein, exerts full heparin binding, cell adhesion, and mitogenic CTGF activity. Recombinant human CTGF is a 11.2 kDa protein of 98 amino acid residues.
- Product Overview
- Human CTGF expressed in E.coli
- CAT# CSC-CTK0589-20 (20 μg); CAT# CSC-CTK0589-100 (100 μg)
- Greater than 95% as determined by SDS-PAGE analysis.
- Determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED50 for this effect is 1.0-2.0 μg/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- CTGF was lyophilized from a sterile-filtered 1 mg/ml solution containing 10 mM Na-acetate buffer (pH 6).
- Please centrifuge product briefly before opening vial. Reconstitute at 0.1 mg/ml with 5 mM Na-acetate (pH 6). This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu CTGF should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
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