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ActoFactor™ Recombinant Human Colony stimulating factor 3 (granulocyte-macrophage) (E. coli)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0151
  • Description
  • Recombinant Human Granulocyte and Macrophage Colony Stimulating Factor (rHu GM-CSF) is a potent species specific stimulator of bone marrow cells. rHu GM-CSF also stimulates precursor cells of granulocytes, macrophages and eosinophils. Human GM-CSF is a 14.48 kDa protein containing 127 amino acid residues.
  • Species
  • Human
  • Product Overview
  • Human CSF3 expressed in E.coli
  • Molecular Mass
  • 14.5 kDa
  • Size
  • CAT# CSC-CTK0151-10 (10 μg); CAT# CSC-CTK0151-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 98% as determined by HPLC and SDS-PAGE analysis.
  • Activity
  • The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is ≤ 0.1 ng/ml, corresponding to a specific activity of Greater than 1 x 10^7 units/mg (WHO standard 88/646, NIBSC).
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized from a sterile protein solution extensively dialyzed against 2 mM sodium phosphate buffer (pH 7.4).
  • Reconstitution
  • Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. For further dilution carrier protein should be added to avoid loss of bioactivity.
  • Storage & Stability
  • The lyophilized powder, though stable at room temperature, is best stored desiccated at -20°C. Reconstituted rHu GM-CSF should be stored in working aliquots at -20°C. Please avoid repeated freeze / thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com