Online Inquiry & Support

USA: 1-631-626-9181   Fax: 1-631-614-7828
Europe: 44-207-097-1828
Email: info@creative-bioarray.com

ActoFactor™ Recombinant Human Colony stimulating factor 3 (granulocyte)

Bookmark and Share
  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0044
  • Description
  • Granulocyte colony-stimulating factor (G-CSF) is a pleiotropic cytokine with an effect on the proliferation, differentiation and activation of granulocyte and primitive hematopoietic progenitors. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Other cell types that can secrete G-CSF include fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Two distinct cDNA clones for human G-CSF encoding 207 and 204 amino acid precursor proteins have been isolated. The recombinant G-CSF contains 175 amino acid residues and has a predicted molecular mass of approximately 18.8 kDa.
  • Species
  • Human
  • Product Overview
  • Human CSF3 expressed in E.coli
  • Sequence
  • A DNA sequence encoding the mature human G-CSF protein generated from the 204 amino acid residue precursor G-CSF isoform1,2 was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0044-5 (5 μg); CAT# CSC-CTK0044-25 (25 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • The biological activity of recombinant human G-CSF is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60. The ED50 for this effect is typically 10 - 60 pg/mL.
  • Endotoxin Level
  • Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
  • Formulation
  • Recombinant human G-CSF is lyophilized from a 0.2 μm filtered solution in 10 mM acetic acid and 50 μg bovine serum albumin per 1 μg cytokine.
  • Reconstitution
  • Reconstitute human G-CSF at a concentration greater than 10 μg/mL with sterile phosphate buffered saline (PBS) containing at least 0.1% human or bovine serum albumin.
  • Storage & Stability
  • Lyophilized human G-CSF is stable for upto twelve months from date of receipt at -20°C to -70°C. Reconstituted human G-CSF can be stored under sterile conditions at 2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Price Inquiry

Please input "bioarray" as verification code.

SEARCH

tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

  

SEARCH

Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
Ordering Information
    • Payment methods we support:
      ✭ Invoice / Purchase Order
      ✭ Credit card
Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com