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ActoFactor™ Recombinant Human Colony stimulating factor 2 (granulocyte-macrophage)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0076
  • Description
  • Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a potent species-specific stimulator of granulocyte-macrophage, eosinophil, megakaryocyte and erythroid progenitors. Human GM-CSF is a 14 kDa protein containing 127 amino acid residues.
  • Species
  • Human
  • Product Overview
  • Human CSF2 expressed in E.coli
  • Sequence
  • A DNA sequence encoding the mature human GM-CSF protein (Ala 18 - Glu 144; Accession #CAA26822) was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0076-10 (10 μg); CAT# CSC-CTK0076-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • The biological activity of recombinant human GM-CSF is measured in a cell proliferation assay using a factor-dependent cell line, TF-1. The ED50 for this effect is typically 6 - 30 pg/mL.
  • Endotoxin Level
  • Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
  • Formulation
  • Recombinant human GM-CSF is lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (PBS) containing 50 μg bovine serum albumin per 1 μg cytokine.
  • Reconstitution
  • Reconstitute human GM-CSF at a concentration greater than 10 μg/mL with sterile PBS containing at least 0.1% human or bovine serum albumin.
  • Storage & Stability
  • Lyophilized human GM-CSF is stable for up totwelve months from date of receipt at -20°C to -70°C. Reconstituted human GM-CSF can be stored under sterile conditions at 2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.

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      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com