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ActoFactor™ Recombinant Human Chemokine (C-C motif) ligand 8

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0083
  • Description
  • Monocyte chemotactic protein-2 (MCP-2) is a member of the b (CC) family of chemokines. It is produced by fibroblasts, peripheral blood mononuclear cells, luteal cells, and MG63 osteosarcoma cells. MCP-2 is a chemoattractant for monocytes, NK cells, T cells, eosinophils and basophils. Human MCP-2 cDNA encodes a 77 amino acid residue protein and it has an apparent molecular mass of 9 kDa.
  • Species
  • Human
  • Product Overview
  • Human CCL8 expressed in E.coli
  • Sequence
  • A DNA sequence encoding the mature human MCP-2 protein was expressed in E. coli.
  • Size
  • CAT# CSC-CTK0083-10 (10 μg); CAT# CSC-CTK0083-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • Activity was determined by ability to chemoattract mouse BaF/3 cells transfected with human CCR5 and the ability to chemoattract THP-1 cells. The ED50 for these effects were typically 0.03 - 0.12 mg/mL and 0.03 - 0.1 mg/mL, respectively.
  • Endotoxin Level
  • Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
  • Formulation
  • Lyophilized from a sterile solution in PBS containing 50 mg of bovine serum albumin per 1mg of cytokine.
  • Reconstitution
  • It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/mL of the cytokine.
  • Storage & Stability
  • Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted human MCP-2/CCL8 can be stored under sterile conditions at 2°to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com