- Both rHu MIP-1 alpha and rHu MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ, with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Recombinant human MIP-1 alpha is a 7.82 kDa single, non-glycosylated polypeptide chain containing 70 amino acid residues.
- Product Overview
- Human CCL3 expressed in E.coli
- CAT# CSC-CTK0459-20 (20 μg); CAT# CSC-CTK0459-100 (100 μg)
- Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
- Fully biologically active when compared to a standard. Activity determined by its ability to chemoattract human blood monocytes using a concentration range of 1.0-10.0 ng/ml.
- Endotoxin Level
- Less than 1 EU/μg.
- Lyophilized from a sterile-filtered protein solution (0.55 mg/ml) with no additives.
- Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted MIP-1 alpha should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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