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ActoFactor™ Recombinant Human Chemokine (C-C motif) ligand 3
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Cat.No.
CSC-CTK0017
Description
MIP-1a is a member of the b (CC) family of chemokines. It is produced by fibroblasts, monocytes, lymphocytes, neutrophils, eosinophils, smooth muscle cells, mast cells, platelets, bone marrow stromal cells, glial cells, epithelial cells, progenitors of dendritic Langerhans cells and several other cell lines. MIP-1a is a chemoattractant for monocytes, T cells, B cells, basophils, eosinophils, neutrophils, NK cells, dendritic cells and osteoclasts. In addition, MIP-1a acts as a stem cell inhibitory factor suppressing proliferation of hematopoietic precursors. Human MIP-1a cDNA encodes a 92 amino acid residue precursor protein containing a 22 amino acid signal peptide. As reported for other chemokines cleavage of the amino terminus domain dramatically reduces its activity. MIP-1a binds to CCR1, CCR4 and CCR5 receptors. The 66 amino acid residues recombinant protein has a predicted molecular mass of approximately 7.5 kDa.
Species
Human
Product Overview
Human CCL3 expressed in E.coli
Sequence
A DNA sequence encoding the mature human MIP-1a protein was expressed in E. coli.
Size
CAT# CSC-CTK0017-10 (10 μg); CAT# CSC-CTK0017-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
Activity was determined by ability to chemoattract cultured human monocytes or BaF/3 cells transfected with the human CCR5 receptor and the ED50 for the chemoattractant activity was typically 2 - 10 ng/mL or 3 - 10 ng/mL, respectively.
Endotoxin Level
Endotoxin level less than 0.1 ng per 1 μg of the cytokine as determined by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA, containing 50 μg of bovine serum albumin per 1 μg of cytokine.
Reconstitution
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 μg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted hMIP-1a/CCL3 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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