Online Inquiry & Support

USA: 1-631-626-9181   Fax: 1-631-614-7828
Europe: 44-207-097-1828
Email: info@creative-bioarray.com

ActoFactor™ Recombinant Human CD40 ligand

Bookmark and Share
  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0289
  • Description
  • Recombinant Human soluble CD40 Ligand (rHu sCD40L/TRAP) belongs to the TNF receptor family and is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and T cells. Binding of CD40L to its receptor, CD40, influences the B cell proliferation and differentiation, and immunglobulin class switching. Creative Bioarray's Human soluble CD40L is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of sCD40L/TRAP.
  • Species
  • Human
  • Product Overview
  • Human CD40LG expressed in E.coli
  • Molecular Mass
  • 16.3 kDa
  • Size
  • CAT# CSC-CTK0289-50 (50 μg); CAT# CSC-CTK0289-250 (250 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.
  • Activity
  • Recombinant human sCD40 Ligand is fully biologically active when compared to a standard. The biological activity of Creative Bioarrays human sCD40L/TRAP was determined by the dose-dependent stimulation of IL-8 production and IL-12 induction by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 5-10 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Lyophilized from a sterile-filtered, concentrated (1 mg/ml) solution in PBS (pH 7.0).
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu sCD40 Ligand should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

Price Inquiry

Please input "bioarray" as verification code.

SEARCH

tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

  

SEARCH

Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
Ordering Information
    • Payment methods we support:
      ✭ Invoice / Purchase Order
      ✭ Credit card
Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com