- CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with ~60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E-cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes. Recombinant human CD4 produced in E. coli contains amino acids 125-202 and is fused with a 4 kDa His-Tag, having a total molecular weight of 19 kDa.
- Product Overview
- Human CD4 expressed in E.coli
- CAT# CSC-CTK0122-20 (20 μg); CAT# CSC-CTK0122-100 (100 μg)
- Greater than 90% as determined by SDS-PAGE analysis.
- Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a protein solution in 1X PBS.
- Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles. Can be used for protein assay, antibody production and Western blotting.
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