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ActoFactor™ Recombinant Human Brain-derived neurotrophic factor

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0006
  • Description
  • Brain-derived neurotrophic factor (BDNF) is a member of the nerve growth factor family of proteins. It binds with high affinity to the TrkB kinase receptor. BDNF has been used to direct human embryonic stem cells (hESC) to neural lineages, and, together with neurotrophin-3 (NT-3) and NT-4, it has been described as a survival factor for hESC1. Mature recombinant BDNF has a predicted molecular mass of approximately 13.6 kDa, with 119 amino acids. The amino acid sequence of mature BDNF is identical in all mammals examined.
  • Species
  • Human
  • Product Overview
  • Human BDNF expressed in Sf21 insect cells
  • Sequence
  • The DNA sequence encoding the BDNF prepropeptide2 was inserted in a baculovirus expression vector and expressed in Sf 21 insect cells.
  • Size
  • CAT# CSC-CTK0006-5 (5 μg); CAT# CSC-CTK0006-25 (25 μg)
  • Expression System
  • Sf21 insect cells
  • Purity
  • Greater than 97% as determined by SDS-PAGE and visualized by silver stain
  • Activity
  • The ED50 of BDNF is typically 3 - 10 ng/mL, as measured by its ability to stimulate proliferation of the TrkB transfected cell line, Baf-TrkB.
  • Endotoxin Level
  • Endotoxin level is less than 1.0 EU/μg.
  • Formulation
  • Lyophilized from a sterile-filtered solution in 0.1 M Sodium Citrate (pH 3.0) and 0.3 M sodium chloride containing 50 μg of bovine serum albumin per 1 μg of BDNF.
  • Reconstitution
  • It is recommended that sterile It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution at no less than 10 μg/mL.
  • Storage & Stability
  • The lyophilized powder is stable for upto 6 months at -20°C to -70°C. Reconstituted BDNF can be stored under sterile conditions at 2 - 8°C for 1 month or at -20°C to -70°C for 3 months in a manual defrost freezer without detectable loss of acti vity. Avoid repeated freezing and thawing.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com