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ActoFactor™ Recombinant Human Bone morphogenetic protein 7

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0533
  • Description
  • The bone morphogenetic proteins are a family of secreted signaling molecules that can induce ectopic bone growth. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (Chen et al., 2004, Kishigami and Mishina 2005). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (Sampath et al., 1992, Kazama et al., 2008), inhibits the branching of prostate epithelium (Grishina et al., 2005), and antagonizes epithelialmesenchymal transition (EMT) (Zeisberg et al., 2003, Yu et al., 2009).In pathological conditions, BMP-7 inhibits tumor growth and metastasis (Buijs et al., 2007), ameliorates fibrotic damage in nephritis (Zeisberg et al., 2003), and promotes neuroregeneration following brain ischemia (Chou et al., 2006). Recombinant BMP-7 has been produced by transient expression of BMP-7 in non-transgenic plant. It consists of a 16.5 kDa single chain with 144 amino residues and contains a 6-His-tag at the N-terminal end. BMP-7 has been purified by sequential chromatography (FPLC) and does not contain animal–derived components or impurities.
  • Species
  • Human
  • Product Overview
  • Human BMP7 expressed in Nicotiana benthamiana
  • Molecular Mass
  • 16.5 kDa
  • Size
  • CAT# CSC-CTK0533-10 (10 μg); CAT# CSC-CTK0533-50 (50 μg)
  • Expression System
  • Nicotiana benthamiana
  • Purity
  • Greater than 97% as determined by SDS-PAGE analysis.
  • Activity
  • The biological activity of BMP-7 is measured by ist ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 was found to be ≤40 ng/ml.
  • Endotoxin Level
  • Less than 0.04 EU/μg.
  • Formulation
  • Lyophilized from a 0.05 M Tris-HCl buffer (pH 7.4).
  • Reconstitution
  • Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com