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ActoFactor™ Recombinant Human Bone morphogenetic protein 4 (HEK)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0528
  • Description
  • BMP-4 regulates similarly to its nearest homologue BMP-2, diverse fundamental processes during embryonic development. BMP-4 and other BMP proteins are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins and have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5 untranslated region of this gene has been described and three variants are described, all encoding an identical protein. Human recombinant BMP-2 produced in HEK cells is a glycosylated disulfide linked homodimer with a total molecular weight of 34 kDa. It has been purified by proprietary chromatographic techniques.
  • Species
  • Human
  • Product Overview
  • Human BMP4 expressed in HEK cells
  • Molecular Mass
  • 34 kDa
  • Size
  • CAT# CSC-CTK0528-10 (10 μg); CAT# CSC-CTK0528-50 (50 μg)
  • Expression System
  • HEK cells
  • Purity
  • Greater than 95% as determined by SDS-PAGE analysis.
  • Activity
  • rHu BMP-4 is fully biologically active when compared to a standard. The specific activity was determined by the dose-dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line) and is typically 2-10 ng/ml.
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated protein solution (1 mg/ml) in 1xPBS.
  • Reconstitution
  • Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into water or other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu BMP-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance:
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com